Chromogenic no special equipment needed for visualization produces dark blue bands. Sensitive detection limit similar to Pierce ECL Western Blotting Substrate. Fade-resistant protein bands stable after membrane drying. Protein Electrophoresis & Western Blotting. Pierce ECL Western Blotting Substrate, Thermo Scientific Supplier: Thermo Scientific A value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. Novex AP Chemiluminescent Substrate should be combined with Nitro Block II for use with nitrocellulose membranes. Features of 1-Step Ultra TMB-Blotting Solution: Fast protein bands visible in less than one minute. Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low-femtogram-level detection by Western blot analysis with horseradish peroxidase (HRP). The emitted light is stable up to 24-96 hours allowing for multiple exposures.Ĭhoose from a standalone substrate, Novex AP Chemiluminescent Substrate or the WesternBreeze Chemiluminescent Immunodetection kits which contains all solutions necessary for your application including blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution, ready-to-use chemiluminescent substrate, wash solutions, incubation trays, pre-cut filter papers, polyester sheet for even substrate development on the membrane. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other. Light emission occurs only during the enzyme-substrate reaction therefore, once the substrate in proximity to the enzyme is exhausted, signal output ceases. The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. CDP-Star is dephosphorylated by AP to yield meta-stable dioxetane phenolate anion intermediate that decomposes and emits light with a maximum intensity at a wavelength of 475 nm. Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.For western blot detection based on alkaline phosphatase (AP), 86 kDa, we offer our CDP-Star substrate that is designed to deliver picogram level sensitivity and is compatible with both traditional x-ray film and CCD-based imaging. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. Pierce ECL Plus Western Blotting Substrate characteristics include: Sensitivity: low. Clarity and Clarity Max ECL Substrates are compatible with any HRP conjugate and ideal for all western blotting. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates. More affordable-high quality and performance at a lower price than other suppliers' similar substrates Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis.More imaging options-X-ray, CCD or laser-based imagers.Longer signal duration-sustained light output for as long as 5 hours.Higher sensitivity-detect targets down to the low-picogram level.Easy to use-can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization.#PI80196X3) through Fisher Scientific and other channels. This product is sold as Pierce ECL 2 Substrate (Cat. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers.
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